List of publications Anna Brunauer

• Journal Articles
• Reviews
• Book chapters
• Conference papers

Journal Articles

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2023

• A. Klebes, H. C. Ates, R. D. Verboket, G. Urban, F. von Stetten, C. Dincer, S. M. Früh
  Emerging multianalyte biosensors for the simultaneous detection of protein and nucleic acid biomarkers
  2023 Biosensors and Bioelectronics, page: 115800

2022

• A. Klebes, A.-S. Kittel, R. D. Verboket, F. von Stetten, S. M. Früh
  Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
  2022 Sensors and Actuators B: Chemical, volume: 355, page: 131283

2021

• A. Brunauer, R. D. Verboket, D. M. Kainz, F. von Stetten, S. M. Früh
  Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay
  2021 Biosensors, volume: 11, issue: 3, page: 74
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  Abstract

  The rapid detection of pathogens in infected wounds can significantly improve the clinical outcome. Wound exudate, which can be collected in a non-invasive way, offers an attractive sample material for the detection of pathogens at the point-of-care (POC). Here, we report the development of a nucleic acid lateral flow immunoassay for direct detection of isothermally amplified DNA combined with fast sample preparation. The streamlined protocol was evaluated using human wound exudate spiked with the opportunistic pathogen Pseudomonas aeruginosa that cause severe health issues upon wound colonization. A detection limit of 2.1 × 105 CFU per mL of wound fluid was achieved, and no cross-reaction with other pathogens was observed. Furthermore, we integrated an internal amplification control that excludes false negative results and, in combination with the flow control, ensures the validity of the test result. The paper-based approach with only three simple hands-on steps has a turn-around time of less than 30 min and covers the complete analytical process chain from sample to answer. This newly developed workflow for wound fluid diagnostics has tremendous potential for reliable pathogen POC testing and subsequent target-oriented therapy.

Reviews

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2021

• H. C. Ates, A. Brunauer, F. von Stetten, G. A. Urban, F. Güder, A. Merkoçi, S. M. Früh, C. Dincer
  Integrated Devices for Non‐Invasive Diagnostics
  2021 Advanced Functional Materials, volume: 31, issue: 15, page: 2010388
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  Abstract

  “Sample‐in‐answer‐out” type integrated diagnostic devices have been widely recognized as the ultimate solution to simplify testing across healthcare systems. Such systems are equipped with advanced fluidic, mechanical, chemical, biological, and electronic components to handle patient samples without any manual steps therefore have the potential to accelerate intervention and improve patient outcomes. In this regard, the combination of integrated devices and non‐invasive sampling has gained a substantial interest to further improve the comfort and safety of patients. In this Review, the pioneering developments in integrated diagnostics are covered and their potential in non‐invasive sampling is discussed. The key properties of possible sample types are highlighted by addressing their relevance for the clinical practice. Last, the factors affecting the transition of integrated devices from academia to the market are identified by analyzing the technology readiness levels of selected examples and alternative remedies are explored to increase the rate of survival during this transition.

Book chapters

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2020

• A. Brunauer, H. Ceren Ates, C. Dincer, S. M. Früh
  Integrated paper-based sensing devices for diagnostic applications
  In: Comprehensive Analytical Chemistry
  2020, Elsevier,
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  Abstract

  Paper-based sensing platforms are one of the most powerful tools for point-of-care diagnostics. The ease of use while ensuring low production costs of such devices is the key for paper-based technologies to find their way to a successful commercialization. Thus, all steps in the analytical process chain from sample interface, sample preparation, signal amplification to signal transduction and data analysis have to be integrated in a single diagnostic platform. In this chapter, we provide a comprehensive and critical overview on recent developments towards integrated paper-based platforms in academia and industry. Herein, innovative solutions are discussed with respect to their sampling methods such as invasive and non-invasive approaches. We also address the requirements and challenges for the sampling and analysis of the different body fluids. Finally, we present our views about the future perspectives, challenges, and opportunities of the integrated paper-based devices.

Conference papers

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2023

• B. Breiner, D. Kainz, S. Wagner, M. Gavage, S. Sahakalkan, R. Marega, F. von Stetten, A. Klebes
  Automated allergen sample preparation and detection via centrifugal microfluidic lateral flow assay
  2023 Eurosensors 2023, Lecce, Italy, 10. - 13.09.2023

2021

• A. Brunauer, A.-S. Kittel, R. D. Verboket, F. von Stetten, S. M. Früh
  Multianalyte-Assays: Simultaneous detection of protein and nucleic acid biomarkers
  2021 European Biosensor Symposium (EBS), online, 09.-12.03.2021
• A. Brunauer, R. D. Verboket, D. M. Kainz, F. von Stetten, S. M. Früh
  Rapid nucleic acid lateral flow immunoassay for the detection of Pseudomonas aeruginosa
  2021 Biosensors 2021, Haeundae-gu, South Korea, (online), 26. - 29.07.2021
• A. Klebes, A.-S. Kittel, R. D. Verboket, F. von Stetten, S. M. Früh
  Simultaneous detection of protein and nucleic acid biomarkers via paper-based multianalyte sensor
  2021 MicroTAS 2021, Palm Springs/USA, 10.-14.10.2021, online

2020

• A. Brunauer, B. Breiner, S. Hennig, D. Kainz, R. Verboket, B. Johannsen, D. Baumgartner, K. Mitsakakis, L. Gutzweiler, Z. Shu, P. Koltay, T. Hutzenlaub, N. Paust, R. Zengerle, F. von Stetten, S. M. Früh
  Actuation principles for bioanalytical platforms to combat infectious diseases
  2020 Virtual EMBL Conference: Microfluidics: Designing the Next Wave of Biological Inquiry 2020, 13.-15.07.2020

2019

• A. Brunauer, F. von Stetten, S.M. Früh
  Sensitive detection of Pseudomonas aeruginosa based on recombinase polymerase amplification combined with an immunological assay
  2019 Molecular Diagnostics Europe, Lissabon/Portugal, 06. - 09.05.2019
• A. Brunauer, B. Breiner, D. Kainz, R. Verboket, F. von Stetten, R. Zengerle, N. Paust, T. Hutzenlaub, S. M. Früh
  Towards digital diagnostic devices - From smart membrane cartridges to highly integrated test stripes
  2019 Diagnostics-4-Future, 27. – 28. 11. 2019, Konstanz