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Bessel light-sheet microscopy: object adapted illumination and opto-genetic switching with computer holograms

Project description

In this project we will develop a novel light sheet microscope, which is equipped with two spatial light modulators (SLM) for high end 4D imaging of large scattering specimen and precise Opto genetic switching. Unprecedented image quality will be achieved by using holographically shaped, object adapted, confocal Bessel beams for imaging various samples provided by the collaboration partners. By a novel postprocessing method, we are able to separate diffusive and ballistic photons, which leads to high image quality also deep inside the specimen. By developing a feedback controlled, holographic multipoint laser illumination through the second SLM, we will realize dynamic and precise Opto genetic switching in large specimen with superior precision and efficiency. Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany´s Excellence Strategy – EXC-2189 – Project ID: 390939984

Start/End of project

01.07.2019 until 30.06.2022

Project manager

Rohrbach A

Contact person

Rohrbach A


Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) im Rahmen der Exzellenzstrategie des Bundes und der Länder – EXC-2189 – Projektnummer 390939984
Benutzerspezifische Werkzeuge