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Publikationsliste Stefanie Reinbold
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Originalarbeiten in wissenschaftlichen Fachzeitschriften Jahre: 2005 |
2004 | alle anzeigen zurück zur Übersicht aller Publikationen Gutmann O, Kuehlewein R, Reinbold S, Niekrawietz R, Steinert CP, de Heij B, Zengerle R, Daub MFast and reliable protein microarray production by a new drop-in-drop technique 2005 Lab on A Chip , Band : 5, Nummer : 6, Seiten : 675 - 681 Gutmann O, Kuehlewein R, Reinbold S, Niekrawietz R, Steinert CP, De HB, Zengerle R, Daub MA highly parallel nanoliter dispenser for microarray fabrication 2004 Biomed Microdevices , Band : 6, Nummer : 2, Seiten : 131 - 137» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We report about the correlation between satellite free droplet release and liquid viscosity in a highly parallel, pressure driven nanoliter dispenser. In extensive studies we found that for liquids of different viscosities the duration of the pressure pulse is the predominant effect compared to pressure amplitude. This result is of essential importance when actuation parameters have to be adopted for different media like oligonucleotide, DNA or protein solutions as it is the case for the non contact high throughput fabrication of microarrays (Ducree et al., 2000). Experiments with oligonucleotides as well as with different proteins showed ascertained carry-over and cross-contamination free printing of DNA and protein microarrays. With it a prime critical point of microarray production is solved, leading to high quality whilst high throughput microarray fabrication. For oligonucleotides printing we found CVs to be better than 1% within one single dispensing channel and 1.5 % within all 24 channels of a 24 channel printhead for each used printing buffer. By optimizing the protein printing buffer the CVs for protein printing were reduced to about 1% within all 24 channels. As a serious practical application test oligonucleotides microarrays were produced using our nanoliter dispenser system. With it a full DNA hybridization experiment was performed. Clear positive signals one hand and no signals in the negative controls on the other hand showed that our system is suited for microarray production. Gutmann O, Niekrawietz R, Kuehlewein R, Steinert CP, Reinbold S, De HB, Daub M, Zengerle RNon-contact production of oligonucleotide microarrays using the highly integrated TopSpot nanoliter dispenser 2004 Analyst , Band : 129, Nummer : 9, Seiten : 835 - 840» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung For the first time we report on the production of oligonucleotide microarrays using a highly parallel and highly integrated, pressure driven TopSpot nanoliter dispenser. The system enables non-contact printing of different media like oligonucleotides, DNA or protein solutions. We optimized the printing buffer needed for oligonucleotides microarrays production with respect to two major aspects: microfluidical optimum for droplet dispensing and biochemical coupling efficiency on different commercially available microarray slides. Coefficient of variations (CVs) of generated spot diameters were measured to be smaller than 1% within one single dispensing nozzle and smaller than 1.5% within all 24 parallel nozzles of the printhead for all printing buffers used. No carry-over and no cross-talk was found, in extensive experiments with oligonucleotides. Optimized printing buffer compositions and concentrations for oligonucleotide microarrays were found, as well as optimized coupling protocols. Furthermore, buffers and protocols were adapted to a host of different microarray slides used. With this system, prime critical points of microarray production are solved, leading to high quality high throughput microarray fabrication.
Konferenzbeiträge Jahre: 2004 | alle anzeigen zurück zur Übersicht aller Publikationen Gutmann O, Kuehlewein R, Reinbold S, Niekrawietz R, Steinert C P, de Heij B, Zengerle R, Daub MFast and reliable protein microarray production by a new drop-in-drop technique 2004 Malmö, Sweden, September 26-30 Proc. MicroTAS 2004 , Seiten : 381 - 383 Kaack RM, Reinbold S, Zengerle R, Daub MIntegrating assay steps on a miniaturized platform: from PCR to hybridization 2004 Malmö, Sweden, September 26-30 Proc. MicroTAS 2004 , Seiten : 436 - 438 Credits: SILK Icons by http://www.famfamfam.com/lab/icons/silk/