Publikationsliste Dr. Lisa Drechsel

• Originalarbeiten in wissenschaftlichen Fachzeitschriften
• Vorträge
• Konferenzbeiträge

Originalarbeiten in wissenschaftlichen Fachzeitschriften

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2015

• L. Drechsel, M. Schulz, F. von Stetten, C. Moldovanc, R. Zengerle, N. Paust
  Electrochemical pesticide detection with AutoDip – a portable platform for automation of crude sample analyses
  2015 Lab Chip, Band: 15, Seiten: 704 - 710
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  Kurzfassung

  Lab-on-a-chip devices hold promise for automation of complex workflows from sample to answer with minimal consumption of reagents in portable devices. However, complex, inhomogeneous samples as they occur in environmental or food analysis may block microchannels and thus often cause malfunction of the system. Here we present the novel AutoDip platform which is based on the movement of a solid phase through the reagents and sample instead of transporting a sequence of reagents through a fixed solid phase. A ball-pen mechanism operated by an external actuator automates unit operations such as incubation and washing by consecutively dipping the solid phase into the corresponding liquids. The platform is applied to electrochemical detection of organophosphorus pesticides in real food samples using an acetylcholinesterase (AChE) biosensor. Minimal sample preparation and an integrated reagent pre-storage module hold promise for easy handling of the assay. Detection of the pesticide chlorpyrifos-oxon (CPO) spiked into apple samples at concentrations of 10−7 M has been demonstrated. This concentration is below the maximum residue level for chlorpyrifos in apples defined by the European Commission.

2014

• A. Kloke, A. R. Fiebach, S. Zhang, L. Drechsel, S. Niekrawietz, M. Hoehl, R. Kneusel, K. Panthel, J. Steigert, F. von Stetten, R. Zengerle, N. Paust
  The LabTube – A novel microfluidic platform for assay automation in laboratory centrifuges
  2014 Lab Chip, Band: 14, Seiten: 1527 - 1537
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  Kurzfassung

  Assay automation is the key for successful transformation of modern biotechnology into routine workflows. Yet, it requires considerable investment in processing devices and auxiliary infrastructure, which is not cost-efficient for laboratories with low or medium sample throughput or point-of-care testing. To close this gap, we present the LabTube platform, which is based on assay specific disposable cartridges for processing in laboratory centrifuges. LabTube cartridges comprise interfaces for sample loading and downstream applications and fluidic unit operations for release of prestored reagents, mixing, and solid phase extraction. Process control is achieved by a centrifugally-actuated ballpen mechanism. To demonstrate the workflow and functionality of the LabTube platform, we show two LabTube automated sample preparation assays from laboratory routine: DNA extractions from whole blood and purification of His-tagged proteins. Equal DNA and protein yield were observed compared to manual reference runs, while LabTube automation could significantly reduce the hands-ontime to one minute per extraction.

Vorträge

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2013

• F. von Stetten, A. Kloke, A. Fiebach, L. Drechsel, S. Zhang, N. Paust, J. Steigert, R. Zengerle
  LabTube - a novel centrifugal microfluidic lab-on-a-chip platform for operation in standard laboratory centrifuges
  2013 Gordon Research Conference on ”Physics and Chemistry of Microfluidics: Challenges, Advances and New Technologies for Diagnostics” in Lucca, Tuscany, Italy , 09. – 14.06.2013

Konferenzbeiträge

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2019

• S. Burger, L. Drechsel, A. Homann, F. von Stetten, R. Zengerle, N. Paust
  LabSlice XL – A centrifugal microfluidic cartridge for the automated bio-chemical processing of industrial process water
  2019 Transducers, Berlin, 23. - 27. 06. 2019

2014

• N. Paust, A. R. Fiebach, L. Drechsel, S. Zhang, R. Zengerle, F. von Stetten
  LabTube - a novel centrifugal microfluidic lab-on-a-chip platform for operation in standard laboratory centrifuges
  2014 IFCC WorldLab Istanbul, 22.-26-06-2014, Istanbul, Türkei
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  Kurzfassung

  The “LabTube” is a novel centrifugal lab-on-a-chip platform that uses a standard laboratory centrifuge as processing device. It automates multistep protocols such as nucleic acid extraction or protein purification, decreasing the hands-on-time from > 6 to 1 minute per extraction. Future applications will include point-ofcare sample-to-answer nucleic acid analysis. The LabTube approach lowers the market entry barrier for microfluidics as the user only requires to purchase disposable parts without the need to invest in specialized processing devices.

2013

• L. Drechsel, M. Schulz, F. von Stetten, R. Zengerle, N. Paust
  Automated on-site detection of organophosphorous pesticides in real food samples
  2013 MicroTAS 2013, Freiburg, 27.-31.10.2013 , Seiten: 1923 - 1925
• A.R. Fiebach, S. Zhang, L. Drechsel, A. Kloke, N. Fritzemeier, D. Wulff, J. Steigert, R. Zengerle, F. von Stetten, N. Paust
  LabTube – an innovative platform for assay automation in laboratory centrifuges
  2013 Microsystemtechnik (MST) Kongress 2013, Aachen, 14. - 16.10.2013 , Seiten: 27 - 30
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  Kurzfassung

  In this paper we present the innovative LabTube platform: A fully automated, easy to handle and cost-effective system that automatizes diverse assays on standard laboratory centrifuges and thus offers a reasonable alternative to existing lab-on-a-chip systems. The LabTube has the size of a 50 ml centrifuge tube and is composed of three stacked revolvers that are operated by a centrifuge-triggered ball-pen mechanism. The upper revolver provides the reagent pre-storage, the middle revolver comprises the assay-specific fluidic unit operations and the lower revolver functions as a unit for the collection and separation of the processed reagents. To demonstrate the functionality of the LabTube on the basis of a frequently used assay, a DNA extraction was performed. The fully automated DNA extraction from rapeseed lysate in the LabTube resulted in a DNA yield of 17,7 +/- 8,1 ng μl -1 in comparison to a manual reference with 14,8 +/- 1,5 ng μl -1. Thus, the hands-on time was reduced from 15 minutes to 1 minute by employing the LabTube, without the need for special training or special laboratory equipment. Currently, the LabTube is also under investigation for protein analysis and for point-of-care diagnostic.
• A. Kloke, L. Drechsel, S. Zhang, A.R. Fiebach, N. Paust, R. Zengerle, F. von Stetten
  The LabTube Platform – Disposable Cartridges For Automated Processing Of Biochemical Assays In Standard Laboratory Centrifuges
  2013 21st annual international BIODETECTION TECHNOLOGIES, Washington, DC, USA, 18-19.6. 2013
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  Kurzfassung

  A laboratory centrifuge can be applied to automate biochemical assays for point-of-care diagnostics or sample preparation such as DNA or protein extraction. Key innovation is integration of liquid handling into a 50 ml centrifuge tube. This "LabTube" harbors three revolvers which are stepwise rotated against each other by a g-force operated ball pen mechanics. The first revolver sequentially releases pre-stored reagents into the second revolver which is equipped with a mixing chamber and a solid phase column. Fractions of processed liquids are collected by the third revolver. Automated LabTube based DNA-extractions showed comparable yields to manual reference extractions.

2012

• R. Zengerle, J. Hoffmann, G. Roth, O. Strohmeier, A. R. Fiebach, L. Drechsel, S. Zhang, A. Kloke, N. Paust, D. Mark, F. von Stetten
  Microfluidic Apps on standard Lab-instruments
  2012 MicroTAS, Okinawa, Japan, 28. Okt. - 01. Nov. 2012 , Seiten: 239 - 241
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  Kurzfassung

  Here we promote a new way of thinking: Designing microfluidic chips that can be processed on standard instruments which are already present in typical laboratory setups. Those instruments could be standard lab centrifuges, real-time PCR cyclers or even second generation sequencers. We call this approach the Microfluidic App approach
• M. Rombach, S. Lutz, C. Dumschat, A. Witt, S. Hensel, S. Frenzel, F. Aßmann, F. Gehring, T. Reiner, H. Drechsel, P. Szallies, D. Mark, G. Roth, R. Zengerle, F. von Stetten
  Sample-to-Answer LabDisk for Point-of-care Analysis of Total Cholesterol from Whole Blood
  2012 MicroTAS 2012, Okinawa/Japan, 28.10. - 01.11.2012 , Seiten: 782 - 784